Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQYQMITREQCLYWDQEDELKKFKDEFALPE-GVIYLDGNSLGARPKKSLAVAQHIISQEWG-EDLINSWN-KADWWGLPTRLGDKVAKLVGAE-QGEVVISDSTTLNLFKVLSAAVKIQADKFPEHKIIVAEKDAFPTDIYIIEGFIDLIQKGYQVELID-------GVEDLSRALEKDVAVVVLSHVNYRTGYFYDMASINEQIHSKDALVIWDLCHSVGAVPMHLNQTDSDFAIGCTYKYLNGGPGSPALLWVNEKHRDQFWQPLSGWWSHKKPFDMAQHYEPANSIRRYLCGTQPVISMSLIECGVDIFLHADMQKIREKSLKLTDLFIQLVHQECSEFGFELITPLDHKHRGSHVSYRHEF--GYEIIQALIARGVIGDYR-----------EPAVLRFGITPLYLGFEDIWNAVQHLKATMLNSEWKNKEYLVRGEVT 5FT6 Chain:A ((6-399)) -------------------PAQFRAQFPALQDAGVYLDSAATALKPEAVVEATQ-QFYSLSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYARP---RLQPGDEIIVSVAEHHANLVPWLMVAQQTGA--KVVKLPLNAQRLPDVDLLPELITPRSRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAFSGHKLY-GPTGI-GVLYGKSELLEAMSPWLGGGKMVH-EVSFD-GFTTQSAPWKLEAGTPNVAGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKR---PGFRSFRC----QDSSLLAFDFAGVHHSDMVTLLAEYGIALRAGQHAQPLLAELGVTGTLRASFAP-YNTKSDVDALVNAVDRALELL--------------

General information: TITO was launched using: RESULT: Template: 5FT6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2013 -25747 -12.79 -69.77 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -12.79 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.481

(partial model without unconserved sides chains): PDB file : Tito_5FT6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FT6-query.scw PDB file : Tito_Scwrl_5FT6.pdb: