Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSFMQQSRLKVILNMRHELREIFIKGCDDKIEKKGDN----------VGLS---FYAFFKNKNDNPELLMEAAHWWIMEHKLDHFEKAVKIKQLVLSEH 3HIM Chain:A ((14-72)) ----------------TSKAAARIRAAAIEVFAAKGYGATTTREIAASLDMSPGAVYPHYKTKES---LLYAISLEGH----------------------

General information: TITO was launched using: RESULT: Template: 3HIM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 114 -3202 -28.08 -69.60 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -28.08 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.634

(partial model without unconserved sides chains): PDB file : Tito_3HIM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HIM-query.scw PDB file : Tito_Scwrl_3HIM.pdb: