Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSVLTMAVNDSPITSVKTTQPLIALYCGSRAGNNPIYREKAIELASGIAEQGFGLVYGGASIGLMGQVAESVLAHGGEAVGVIPEFMLDYEVAHAGLTELHVVETMHERKAMMAERASAFIALPGGLGTFEEILEIATWGQLNQHQKPMILYNVNGFYNALIAQLDHAVQEGFLPLQHRAKLIVCEEIDQIYNVIKNLKMPKRFVV 1T35 Chain:E ((1-184)) --------------------MKTICVFAGSNPGGNEAYKRKAAELGVYMAEQGIGLVYGGSRVGLMGTIADAIMENGGTAIGVMPSGLFSGEVVHQNLTELIEVNGMHERKAKMSELADGFISMPGGFGTYEELFEVLCWAQIGIHQKPIGLYNVNGYFEPMMKMVKYSIQEGFSNESHLKLIHSSSRPDELIEQMQNYSYPIL---

General information: TITO was launched using: RESULT: Template: 1T35.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 866 -118953 -137.36 -646.48 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain E : 0.78 3D Compatibility (PKB) : -137.36 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_1T35.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1T35-query.scw PDB file : Tito_Scwrl_1T35.pdb: