Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRIALLDYGMGNLHSAAKALEYVGATVDVTNDPKLIAQADKIVFPGVGAMRDCMQGMREAGIDEVVRK-AAFNKPVLAICVGMQALLQSSEENGGVDALGIFDGVVKHFPQMEGLKVPHMGWNQVHQMDPSHPMWNNIEQDARFYFVHSYYVEPKDENLVAATCEY-GVNFCTAIHKDNLFATQFHPEKSHTAGLQLLKNFVEWNI 1KA9 Chain:H ((3-196)) -MKALLIDYGSGNLRSAAKALEAAGFSVAVAQDPKAHEEADLLVLPGQGHFGQVMRAFQESGFVERVRRHLERGLPFLGICVGMQVLYEGSEEAPGVRGLGLVPGEVRRFRA---GRVPQMGWNALEFGG----AFAPLTG--RHFYFANSYYGP-LTPYSLGKGEYEGTPFTALLAKENLLAPQFHPEKSGKAGLAFLALARRY--

General information: TITO was launched using: RESULT: Template: 1KA9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 1092 -54791 -50.17 -285.37 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain H : 0.79 3D Compatibility (PKB) : -50.17 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.494

(partial model without unconserved sides chains): PDB file : Tito_1KA9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KA9-query.scw PDB file : Tito_Scwrl_1KA9.pdb: