Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKGYEVNFDGLV-GPTHHYAGLSFGNEASTKNCNNLSNPKLAAKQGLLKMKALADMGMKQGVLAPHERPHVPMLRRLGFTGDDISVVAQAMR-YSPELLSSLSSASPMWTANAATVSPSADSQDERVHFTAANLNNKFHRSIEAETTSQVLQAIFKNERHFVHHEALPQVALFGDEGAANHNRLGGDYAKRGVQVFVYG-------QQHLNNGLPGPKRYPARQTREASEAIARLHRLDESH---TVFVQQNPDVIDQGVFHNDVIAVSNQQVSFHHQHAFLNQDQAFAEIRQKMASIGEDFISIEVPENRVTVDDAVATYLFNSQILTRPDGGMTIVVPEESRQNAAVWS------YLNDMIQMGTPIDAIQVYDLRESMRN--GGGPACLRLRVAL-----NETELNAVNPKVLMNDQLFMTLNQ-WVDKHYRDRLAQ--EDLADPHLLMESRMAFDELTKILGLGSVYPFQK---------------------- 4OPU Chain:A ((2-453)) M--KELKYDVLIIGGG--FAGSSAAYQLSRRG----------LKILLVDSKPWNRIGDKPCGDAVS-KAHFDKLGMPYPKGEELENKINGIKLYSPDM-------QTVWTVNHEGFELNAPLYNQRVLKEAQD------RGVEIWDLTTAMKPIFEDG--YVK-------------GAVLFNRRTNEELTVYSKVVVEATGYSRSFRSKLPPELPITEDLDDKDADVAYREVLLTKEDIEDHDYLRIFIDQETSPGGYWWYFPKGKNKVNVGLGIQGGMGYPSIHEYYKKYLDKYAPDVDKSKLLVKGGALVPTRRPLYTMAWNG-IIVIGDSGFTVNPVHGGGKGSAMISGYCAAKAILSAFETGDF-SASGLWDMNICYVNEYGAKQASLDIFRRFLQKLSNDDINYGMKKKIIKEEDLLEASEKGDLHLSVADKAMRVISGLGRPSLLFKLKAVAESMKKIKELYLNYPRSPSSLGSWRREVDNVLTEFNKSLS

General information: TITO was launched using: RESULT: Template: 4OPU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2418 64561 26.70 160.60 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 26.70 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.245

(partial model without unconserved sides chains): PDB file : Tito_4OPU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OPU-query.scw PDB file : Tito_Scwrl_4OPU.pdb: