Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MHIYILGSAAGGGCPQWNCNCPNCHGVRTGTINAKVRTQSSIAISENGVDWILLNASPDIRQQLFDFKAAQPARKLRDTGITNVILMDSQLDHTTGLLTLR--EGCPMNVWCTEMVYQDLTTGFPVFNMLKHWNGGLQYHQV-DPKQAFIIDGFENLEFLPLIIQSAAPPYSPHRHDPHEGDNIALIIKDHKTQKQLFYAPGLGKIDDQIMQIMQDS--DCVMIDGTLWTDDEMQQTGVGKKTGREMGHLYISGEGGSLSYLNQLSTPKKVLIHINNTNPILNEDSAQFAELKANGVEVAFDGMQIEL 3G1P Chain:A ((2-250)) SLTLTLTGTGGAQGVPAWGCECAACARARRS--PQYRRQPCSGVVKFND------------AITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPPDEQG--------CDDLFKHPGLLDFSHTVEPFVVFDLQG---LQVTPLPL-------------NHSKLTFGYLLETAH--SRVAWLSDTAGLPEKTLKFLRNNQPQVMVMDCS--------------HPPRADAPRNHCDLNTVLALNQVIRSPRVILTHIS-----HQFDAWLMENALPSGFEVGFDGMEIG-

General information: TITO was launched using: RESULT: Template: 3G1P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1409 21352 15.15 87.87 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 15.15 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.373

(partial model without unconserved sides chains): PDB file : Tito_3G1P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G1P-query.scw PDB file : Tito_Scwrl_3G1P.pdb: