Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNIYQFEAELLVGDIKQFADYKGKVLLIVNTASKCGFTP-QFAGLEKLYEKYKDLGLEVLGFPCNQFGGQDPGSNKEIGTFCQRN-YGVKFPMFAKVDVKGPEAHVIFRYLTREAKGILGSSTIKWNFTKFLVGKDGSVLNRYAPTTKPEALEADIEKALAS 2P31 Chain:B ((27-180)) -QDFYDFKAVNIRGKLVSLEKYRGSVSLVVNVASECGFTDQHYRALQQLQRDLGPHHFNVLAFPCNQFGQQEPDSNKEIESF-ARRTYSVSFPMFSKIAVTGTGAHPAFKYLAQ---TSGKE--PTWNFWKYLVAPDGKVVGAWDPTVSVEEVRPQITALV--

General information: TITO was launched using: RESULT: Template: 2P31.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 796 -22012 -27.65 -144.82 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -27.65 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.570

(partial model without unconserved sides chains): PDB file : Tito_2P31.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P31-query.scw PDB file : Tito_Scwrl_2P31.pdb: