Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPIYNAPLADMKFILNDVFKAEQFWQANEKLAHVDAATAEAILEEMAKFAQNVTHPLNRTGDEEGARYENGEVFTPAGFKDAFRQYAEGGWIGLGADEEWGGQGMPKMLTVLADEMLFATNPSFMLYPLLSV-GAGMALSSYASQEQKETYLPKIYSGEWSGTMCLTEPHAGTDLGIIKTKAERNE-DGTYNITGTKIFITGGDNDLAENIIHLVLAKTPDAPAGSRGISLFIVPKYLVNEDGSLGDRNHVGPGSIEHKMGIKASATCVMNFDG---AKGYLVGKENEGLAAMFVMMNYERLSMGIQGLGASEFAYQNAAQYATARLQGRSAAGVQSPSKPADSILVHGDVRRMLLNVRANNEASRAFAVYVGQQLDITKFSTD-AEAVKKANDRVALLTPIAKAYLTDTAFQATLDAQMVFGGHGYIREWGMEQCIRDLRIAQIYEGTNGVQSQDLIGRKTIKCGGAFIAEYITEIRDFANDLDTDLNFIKDA--TLDAATEIEA----ITQFIIEQAAENVEFPNSAAVDYLHAVGLLSFSYMFAKIAAAAKDKSGDFYQNKLALAQYFVERILPDIDARIAKIKAGSDLIMGFSEDYFTNQA 2Z1Q Chain:B ((27-526)) ---------------------------------DFDESVKEIARTTRTFVEREVLPLLERMEHGELEL-------N---VPLMRKAGELGLLAIDVPEEYGGLDLPKVISTVVAEELS-GSGGFSVTYGAHTSIGTLPLVYFGTEEQKRKYLPKLASGEWIAAYCLTEPGSGSDALAAKTRATLSEDGKHYILNGVKQWISNAGF--AH--LFTVFAKVDG-----EHFTAFLVERDT----------PGLSFGPEEKKMGIKASSTRQVILEDVKVPVENVLGEIGKGHKIAFNVLNVGRYKLGAGAVGGAKRALELSAQYATQRVQFG------------RPIGRFGLIQQKLGEMASRIYAAESAVYRTVGLIDEALLGKKGPEAVMAGIEEYAVEASIIKVLGSEVLDYVVDEGVQIHGGYGYSQEYPIERAYRDARINRIFEGTNEINRLLIP-GMLLRRA-------------------------EPEDLELHQVQNLKKLALMVAGLAVQKYGQGVEEEQEVLGAVADILIDAYAAESA--LLR-ARR----LGGLAPVLARIYLAQALDRAQAGAL---------------------

General information: TITO was launched using: RESULT: Template: 2Z1Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2550 -46791 -18.35 -100.84 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : -18.35 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.512

(partial model without unconserved sides chains): PDB file : Tito_2Z1Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z1Q-query.scw PDB file : Tito_Scwrl_2Z1Q.pdb: