Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMLIRKAKKDDTDTIVAIIAPYVDQVIANDEGRSRFKSEAIQTIFDREDIHYFVGEIDQQIVGIVAYME-----PSHLMHFFIKKTHLKLGLGRQLWDFIEEKIKNEN-IDIEKITVNS-SFYAQDIYEKFGFIVSGDAAEK--WG--IRFIPMTKYYALASEK 4L8A Chain:A ((3-161)) MQLSHRPAETGDLETVAGFPQDRDELFYCYPKAIWPFSVAQLAAAIAE-RRGSTVAVHDGQVLGFANFYQWQHGDFCALGNMMVAPAARGLGVARYLIGVMENLAREQYKARLMKISCFNANAAGLLLYTQLGYQPRAIAERHDPDGRRVALIQMDKPLE-----

General information: TITO was launched using: RESULT: Template: 4L8A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 675 -55536 -82.28 -375.24 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -82.28 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.410

(partial model without unconserved sides chains): PDB file : Tito_4L8A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4L8A-query.scw PDB file : Tito_Scwrl_4L8A.pdb: