Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQFKYSLGVISLCSALFLVGCNDDNSSVSQTPTVQEQKQKLQPIIIQGALPVESERMASKLENKTVETIGGWTFWKGTYNGYPMIISKTRMGMSNSAAATALAIERYKPIAIINQGTAGGHDPDLHVYDIVLGKYATNIGAFRTPKQPLGGGSNSLTWVEAFDVLPTKESDPEPIAIRKFEGDQELLMAAHKVR-----YDKGEVVEGTIGSADVWNNELDRIQFFHERY-GTSVEEMEAVSVAQIASQFNVPFLGIRILSNNITN--NGAYDPGT---GEACQDYVLNVAEE 5DK6 Chain:A ((19-255)) -------------------------------------YFQSMKAGIIGAMEPEVAILKEKLTDAKSTEHAGYTFHQGQLDGSDVVIVQSGIGKVAAALATAILIDRFQVDYVVNTGSAGGFDASLKVGDIVVSSEVRYHDVDLTAF-----GYEIGQ-------LPAN--------PAAFMPHDDLVAAAKKGIEQLSQ--NIKAVTGLITTGDTFMTKEEDVAKARANFPTMAAVEMEGAAIAQACLQLKTPFVVIRSLSDIAGKESPHTFEEYLETAAVNSSQLVLNMLG-

General information: TITO was launched using: RESULT: Template: 5DK6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1272 -56322 -44.28 -253.70 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -44.28 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.516

(partial model without unconserved sides chains): PDB file : Tito_5DK6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DK6-query.scw PDB file : Tito_Scwrl_5DK6.pdb: