Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKFQPIINQIKEKEHQQKIKDLFAKI-TETYPQLDTTVKWNQP-MFTDHGTFII-AFSFAKNHFSIAPEKAAI-RALEKNIQEAGYVYTDNVIKVPWKSVINWELIEQLISFNIEDKKGHDKFWR- 2I8D Chain:A ((3-123)) -GSLAEWYQRIPT-PDDLTRVESLFANMQAQFPQLKLEFKWNQPMFTD--HGTFIMGFNPSKKHLAVAIEPQ-TMTRFIPQIDKAGYDHSQI-IRFPWHKPLDEQLIHDLIAYTIDQKKDATTFWQR

General information: TITO was launched using: RESULT: Template: 2I8D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 242 3299 13.63 28.44 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 13.63 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.314

(partial model without unconserved sides chains): PDB file : Tito_2I8D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I8D-query.scw PDB file : Tito_Scwrl_2I8D.pdb: