Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIFSERMGEMTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKSTLLKSFTRILPVKSGKIIVNNSDISTYSSKE-------------LAKKIALLAQTSEHPTGMTVEEVVSYGRYPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIAT----LSGGQTQRVWIAMALAQETDILILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKLITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL 1B0U Chain:A ((5-241)) ----------NKLHVIDLHKRYGGHEVLKGVSLQARAGDVISIIGSSGSGKSTFLRCINFLEKPSEGAIIVNGQNINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFNLWSHMTVLENVMEA--PIQVL--GLSK--HDARERALKYLAKVGIDERAQGKYPVHLSGGQQQRVSIARALAMEPDVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVT-HEMGFARHVSSHVIFLHQGKIEEEGDPEQVF----------------------------------------

General information: TITO was launched using: RESULT: Template: 1B0U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1116 -40694 -36.46 -184.97 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -36.46 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.472

(partial model without unconserved sides chains): PDB file : Tito_1B0U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1B0U-query.scw PDB file : Tito_Scwrl_1B0U.pdb: