Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPYQPRKTFEEVSLQELANSIEQSGV-FQPIIVRRS-AVKGYEIIAGERRFRASKLAEKETIPAIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPYIANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLEQLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEYLSQKDLTRILDILNIQFDEE 5X0B Chain:A ((14-94)) --------------------------KKVDYVFIELDKMKPHE-----QLVQKELEAFIESVTGSGIFWKPMLLAKVPGEDMYLIVDGHHRWAGLQKLGAKRAPSVILDYFS------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5X0B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 301 -23840 -79.20 -301.77 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -79.20 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.545

(partial model without unconserved sides chains): PDB file : Tito_5X0B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5X0B-query.scw PDB file : Tito_Scwrl_5X0B.pdb: