Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFGGMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPLYFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAVGVAEKSQIIDGSKVVEGDVLLGLVSSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLKEVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFA-DDLAAEIDESKVPVLPIFKALEKYGQIKHEEMFEIFNM-GVGLMLAVSPENVERVKELL-DEAVYEIGRIVKKENESVIIK 5CM7 Chain:A ((12-292)) --------------------FSIIDQYFNRQSHPDVALGIGDDSALITPPP----NQQLVICADTLVAGRHFPLET-SPHAIGWKSVAVNLSDIAAMGAKPHSILLAISLPQVDHEWLEGFSQGIYDCCNQFGVALIGGDTTQGP------HLTITVTAMGWIETGKAVLRSGAKVGDYVCVSGQIGDAAYGLQHLGHSL-----------------QQRLDYPTPR-CKLGEEL-K-GLASSMIDVSD-GLAQDLGHILKASKVGARLILEKLPVDPVLQQIEEQQRWQ---YA-LAGGDDYELCFTITPQNYEKLLQKQLDVKITMIGQIVEQTK------

General information: TITO was launched using: RESULT: Template: 5CM7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1572 -143573 -91.33 -516.45 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -91.33 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.391

(partial model without unconserved sides chains): PDB file : Tito_5CM7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5CM7-query.scw PDB file : Tito_Scwrl_5CM7.pdb: