Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEIDRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLI-GYGVEQIISTGTCGVLAD-IEENAFLVPVRALRDEGASYHYVAP-CRYMEMQPEAIAAIEEVLEDRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELLFTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL 4MAR Chain:B ((28-254)) ------------------------SAPPGAVAEAILLPGDPLRAKYIAENFLENPVL--YNQVRNMFGYTGTYKGKRVSVQGTGMGIPSASIYIHELVQFYGCKTLIRVGTAGAITERLKLRDLVIAQAACTDSSINNLRFAGQNYAPIATFDLLRRAYEQAQSRGMPVHVGNVLSTDTFYHDQPNPYQLWAQFGVLAVEMEAAGLYTLAAKFGVQALCILTISDHLITGEKTTPQERQ-ETFDQMIEVALETI---

General information: TITO was launched using: RESULT: Template: 4MAR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1275 -69799 -54.74 -311.60 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : -54.74 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.540

(partial model without unconserved sides chains): PDB file : Tito_4MAR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MAR-query.scw PDB file : Tito_Scwrl_4MAR.pdb: