Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADEGINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLEDNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRNFRELP------------QWVR--------------DNKE-K-FMDKRVVVYC-TGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKDPEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILTSEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV 3G5J Chain:A ((5-132)) ------------------------------------------------------------------------------------------------------------------------SVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNEHNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGSIVNLLSSLGV-NVYQLEGGYKAYRNFVL------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3G5J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 295 -10978 -37.21 -110.89 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -37.21 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.327

(partial model without unconserved sides chains): PDB file : Tito_3G5J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G5J-query.scw PDB file : Tito_Scwrl_3G5J.pdb: