Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSLRFQSVFDIIGPVMIGPSSSHTAGA-VRIGKIVSSIFD----DTPTEVEFQLFNSFAKTYRGHGTDLALVAGILGM---DTDDPE-IP--------NSLEIAHKRGI----KIVWTIQKDSNAPHPNTT-KITV--KNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDIPGMIALVTEALSRYGINIAQM-NVTREKAGEKAIMIIEVDSRNCDEAIEEIRKI-PHLHNVNFFK------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ 4RQO Chain:A ((20-476)) -----NISVFDLF-SIGIGPSSSHTVGPMLAANAFLQLLEQKNLFDKTQRVKVELYGSLALTGKGHGTDKAILNGLENKAPETVDPASMIPRMHEILDSNLLNLAGKKEIPFHEATDFLFLQKELLPKHSNGMRFSAFDGNANLLIEQVYYSIGGGFITTEE------DFDKNPPPYPF--ATATELLKLCKKH----HLTIAELMLVNEKTWR---------STAEIHKGILDIAKVMDDCINNGCKHDGVLPGGLNLKRRAPDLYRKLIFEQSDIMNHLNLYAMAVNEENAAGGRIVTAPTNGAAGIIPAVLKYCQQAHDRMSNEDIYTYFLTAAAIGILYKKGASISGAEVGCQGEVGVASSMAAAGLTAVLGGTIEQVENAAEIAMEHHLGMTCDPVLGLVQIPCIERNAMGAVKAVNATRMALIGDGQHQISLDKVIKTMKQTGMDMQSIYKETSMGGLAVNLPEC

General information: TITO was launched using: RESULT: Template: 4RQO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 892 3776 4.23 19.27 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 4.23 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.373

(partial model without unconserved sides chains): PDB file : Tito_4RQO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RQO-query.scw PDB file : Tito_Scwrl_4RQO.pdb: