Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------------------------------------------------------------------------------------MAKLEVKDNKKLVLKSVICKKLHDTKVEDVDQEINRFHQHLQLLKAQIFGPLIVKSCGTTIHDDGLIT-TDFEFYIQAHNAQQYSNIYDVQDSISVPYCLYVRFEDSPEYLQYAYSKLDLYVYENDIQTDGIVYTVYV-----NSSPEKMVVDIFIPIVSL 1EXJ Chain:A ((3-277)) ESYYSIGEVSKLANVSIKALRYYDKIDLFKPAYVDPDTSYRYYTDSQLIHLDLIKSLKYIGTPLEEMKKAQDLEMEELFAFYTEQERQIREKLDFLSALEQTISLVKKRMKRQMEYPALGEVFVLDEEEIRIIQTEAEGIGPENVLNASYSKLKKFIESADGFTNNSYGATFSFQPYTSIDEMTYRHIFTPVLTNKQISSITPDMEITTIPKGRYACIAYNFSPEHYFLNLQKLIKYIADRQLTVVSDVYELIIPIHYSPKKQEEYRVEMKIRIA--

General information: TITO was launched using: RESULT: Template: 1EXJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 327 11837 36.20 77.36 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : 36.20 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.326

(partial model without unconserved sides chains): PDB file : Tito_1EXJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EXJ-query.scw PDB file : Tito_Scwrl_1EXJ.pdb: