Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQSYKNIEVIIIDDNYSNVNLRNKIIHQ--FGYT-NHRIKLILSNEDLGATNARNIGIKNSRGKYISFLDDDDEYMPDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIAGTSFWLCKKEVLELINGFEK-IDSHQDGVVLLKLLAQGYQIDIVREFLVNYYAHSKENGITGVTQKTINADEEYYNYCRKYFNLLSFNERILVTKKYYSLNIKRLLLIGDKCKALKVIKKAREEKIFNEFLFLKYMLLYNFSFFYCIYDNYVQLKFRK 2Z86 Chain:B ((372-581)) ----KRVPLVSIYIPAYNCSKYIVRCVESALNQTITDLEVCICDDGSTDD-----TLRILQEHYANHPRVRFIS-QKNKGIGSASNTAVRLCRGFYIGQLDSDDFLEPDAVELCLDEFRKDL--SLACVYTTNRNIDREGNLISNGYNW-PIYSREKLTSAMICHHFRMFTARAWNLTEGFNESISNAVDYDMYLKLSEVG-PFKHINKICYNRVLHG-NTSIK-------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2Z86.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1143 -43516 -38.07 -212.27 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -38.07 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.373

(partial model without unconserved sides chains): PDB file : Tito_2Z86.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Z86-query.scw PDB file : Tito_Scwrl_2Z86.pdb: