Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQV---GLKEVANYLGEIFKRVGAE-VEIDES------YT------APFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVWTEDPFTLSVR--NGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESASTDLDKYLEKHAD-KLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDI--HSSYGG-VVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEEVSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVRLVPGLEPHDVLEKIRKQLDKNGF-D----KVELYYTLGEMSYRSDMSAPAILNVIELAKKFYPQGVSVLPTTAGTGPMHTV-FD-ALEVPMV--AFGLGNANSRDHGGDENVRIADYYTHIELVEELIRSYE 4G1P Chain:A ((4-475)) -----SLTSVFQKID-SLKPQFFSRLTKAIQIPAVSSDESLRSKVFDKAKFISEQLSQSGFHDIKMVDLGIQPPP-STPNLSLPPVILSRFGSD-PSKKTVLVYGHYDVQPAQLEDGWDTEPFKLVIDEAKGIMKGRGVTDDTGPLLSWINVVDAFKASGQEFPVNLVTCFEGMEESGSLKLDELIKKEANGYFKGVDAVCISDNYWLGTKKPVLTYGLRGCNYYQTIIEG--PSADLHSGIFGGVVAEPMIDLMQVLGSLVDSKGKILIDGIDEMVAPLTEKEKALYKDIEFSV-EELNAATGSKTSLY-DKKEDILMHRWRYPSLSIHGVEGAFSAQGAKTVIPAKVFGKFSIRTVPDMDSEKLTSLVQKHCDAKFKSLNSPNKCRTELIHDGAYWVSDPFNAQFTAAKKATKLVYGV-DPDFTREGGSIPI-TLTFQDALNTSVLLLPMGRG--DDGAHSINEKLDISNFVGGMKTMAAYLQYY-

General information: TITO was launched using: RESULT: Template: 4G1P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2287 75833 33.16 172.35 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 33.16 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.466

(partial model without unconserved sides chains): PDB file : Tito_4G1P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G1P-query.scw PDB file : Tito_Scwrl_4G1P.pdb: