Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSYRIYSDMFDF------AVDL------RPNPDYSLIQETIALMGDFLAVRGQNLRPFSLAIYGKMEREGKKFGLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIAAEDLVLYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKE-VAVSSHMVQQIKQNINQNFLTSSKETVVSLVEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQLKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDKS 1KKH Chain:A ((6-315)) -MIIETPSKVILFGEHAVV-YGYRAISMAIDLTSTIEIKETQEDEIILNLNDLNKSLGLNLNEIKNINPNNFGDFKYCLCAIKNTLDYLNI--EPKTGFKINISSKIP---ISCGLGSSASITIGTIKAVSGFYNKELKDDEIAKLGYMVEKEIQGKASITDTSTITYKGILEIKNNKFRKI----KG---------------EFEEFLKNCKFLIVYAEKRKKKTAELVNEVAKIEN--KDEIFKEIDKVIDEALKIKNKEDFGKLMTKNHELLKKLN--ISTPKLDRIVDIGNRFGFGAKLTGAGGGGCVIILVNEEKE-KELLKELNKEDVRIFNCRM-------

General information: TITO was launched using: RESULT: Template: 1KKH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1685 8886 5.27 29.92 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 5.27 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.381

(partial model without unconserved sides chains): PDB file : Tito_1KKH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KKH-query.scw PDB file : Tito_Scwrl_1KKH.pdb: