Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIENKVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKELKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLAL---PEVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDIGYDV 2GM8 Chain:C ((11-219)) ------------VTGELRRRADGIWQRILAHPFVAELYAGTLPMEKFKYYLLQDYNYLVNFAKALSLAASRAPSVDLMKTALELAYGTVTGEMANYEALLKEVGLSLRDAAEAEPNRVNVSYMAYLKSTCALEGFYQCMAALLPCFWSYAEIAERHGGKLRENPVHVYKKWASVYLSPEYRGLVERLRAVLDSSG---LSAEELWPYFKEASLYELEFWQAAYE-

General information: TITO was launched using: RESULT: Template: 2GM8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 916 -107850 -117.74 -523.54 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain C : 0.68 3D Compatibility (PKB) : -117.74 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.516

(partial model without unconserved sides chains): PDB file : Tito_2GM8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GM8-query.scw PDB file : Tito_Scwrl_2GM8.pdb: