Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKFSPLCWIVPSIYVIMKEGFKIGKGVFMLKLGVI-GTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFASRY---QNIQLFDQLEVFFKS-SFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVS-QPQEWFDLIQTAEKNNCFI-FEA-ARNYHEKAFTTIKNFLADKQVLG-ADFNYAKYSSKMPDLLAGQTPNVFSDRFAGGALMDLGIYPLYAAVRLFGKANDATYHA----QQLDNSIDLNGDGILFYPDYQVHIKAGKNITSNL-PCEIYTTDGTLTLNTI------EHIRSAIFTDHQGNQVQLPIQQAPHTMTE------EVA-AFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK-- 1YDW Chain:B ((6-360)) ----------------------------QIRIGV-MGCADIARKVSRAIHLAPNATISGVASRSLEKAKAFATANNYPESTKIHGSYESLLEDPEIDALYVPLPTSLHVEWAIKAAEKGKHILLEKP-VAMNVTEFDKIVDACEANGVQIMDGTMWVHN--PRTALLKEFLSDSERFGQLKTVQSCFSFAGDEDFLKNDIRVKPGLDGLGALGDAGWYAIRATLLANNFELPKTVTAFPGAVLNEAGVILSCGASLSWEDGRTATIYCSFLANLTMEITAIGTKGTLRVHDFIIPYKETEASFTTSTKAWFNDLVTAWVSPPSEHTVKTELPQEACMVREFAIKNNGAKPDGYWPSISRKTQLVVDAVKESVDKNYQQISLS

General information: TITO was launched using: RESULT: Template: 1YDW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1714 39639 23.13 123.48 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : 23.13 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.382

(partial model without unconserved sides chains): PDB file : Tito_1YDW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YDW-query.scw PDB file : Tito_Scwrl_1YDW.pdb: