Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLIQLYLLKLELFWFELHQHYHFSEPQKILNELIAAYSAAYSEKQRAYHTVQHLYECLSLIETVQSE--------LNDPYAVALALWFHDVVYEPQA------------------LDNELKSAELFEQLMAQ-DL------Q----LDTMQKIKRWILATQKHA-----------------------PTDETDLQFLLDIDLAILAATPERFMQYEQQIQQEYSWADPEVYSIKRKEVLMNFYQSEPLYQTAYFQKNFELNAKQNLKKILE 5JO3 Chain:B ((73-254)) ----------------------------------------YRK-NVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHPGVSNQFLINTNSELALMYNDESVLEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFNLEDPHEKELFLAMLMTACDLSAITKPWPIQQRIAELVATEF------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5JO3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 420 7286 17.35 59.72 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.56 3D Compatibility (PKB) : 17.35 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_5JO3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JO3-query.scw PDB file : Tito_Scwrl_5JO3.pdb: