Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSDSSNQLMPLSLSAPGVNLGAYISTVNQIPILTAEQEKELAERYYYDQDLDAAKMLVMSHLRFVVHIARSYAGYGLPQGDLIQEGNLGLMKAVKRFDPNMGVRLVSFAVHWIKAEIHEYVIRNWRIVKIATTKAQRKLFFNLRSLKKSSKK--LTLEEAQSIANDLNVTPEQVLEMEGRLTAYDAAFEAQGDDDDDTPHTAPVLYLEDN-RYDPARLVENEDWEEQSTSALHDAMNQLDDRSRNILQRRWLDD--DKSTLHELAAEYNVSAERIRQLEKNAMEKIKVAMSAS 5IPM Chain:F ((53-319)) --------------RVLDATQLYLGEIGYSPLLTAEEEVYFARRA-LRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVK--ELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLN-ERITSVDTPLGGDSEK---ALLDILADEKENGPEDTTQDDDM----KQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQT-

General information: TITO was launched using: RESULT: Template: 5IPM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 791 -30256 -38.25 -115.48 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain F : 0.73 3D Compatibility (PKB) : -38.25 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_5IPM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IPM-query.scw PDB file : Tito_Scwrl_5IPM.pdb: