Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKMLAQLQNRFDQWVEQIVQYLDRLSVRERIMVVFTTIFVVVVIVGYSLWKMHSLAEQQQKRLNDLKDLMVWMQSNAVTMKPANELELDKSGKIQRVAQQQGLTVSSQQNGEQ--LQIVVTHQNYAILANFLIQLA---QMGLSIQKMEMVSSEGQIKLTATV 2GV1 Chain:A ((16-79)) --------------------------------------------------------------------------------------GVGFRYTTQYEAKRLGLTGY-AKNLDDGSVEVVAC-GEEGQVEKLMQWLKSGGPRSARVERVLSEP-----------

General information: TITO was launched using: RESULT: Template: 2GV1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 161 -648 -4.02 -10.97 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -4.02 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_2GV1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GV1-query.scw PDB file : Tito_Scwrl_2GV1.pdb: