Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLDIHLNFIKRLSSMNKEIFKIINPSTLYDPTPNAYSHVAVVSNFNNIIHIAGQGGEDQQGQLS-SHFSEQVLQTFENINYALVAANAELTDIAVLRILVVDHSVEKHEILIKIMKDLWKNHPFPACTLIPVPRLALEHMLIEVEATAYT 2CVL Chain:B ((4-123)) -----------------------VKTDRAPAA-IGPYAQAVKA---GGFVFVSGQIPLAPDGSLVEGDIRVQTERVMENLKAVLEAAGSGLSRVVQTTCFLADMED--FPGFNEVYARYFTP-PYPARATVAVKALP-RGVRVEVACVALA

General information: TITO was launched using: RESULT: Template: 2CVL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 588 -41386 -70.38 -347.78 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : -70.38 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.391

(partial model without unconserved sides chains): PDB file : Tito_2CVL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CVL-query.scw PDB file : Tito_Scwrl_2CVL.pdb: