Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTISHIDPEYQANTIEPSVQQDWENRKVFKVADTVEGKHRYILSMFPYPSGKLHMGHVRNYTIGDVISRFYRLKGETVLQPMGWDAFGLPAENAAIAHKVAPAKWTFENIAYMRDQLKKLGLSVDWDREFATCTPEYYHWEQWLFVQLYKKGLIYRKLSTVNWDPVDQTVLANEQVENGRGWRSGALVEKRDIPMYYFRITDYAQELLDDLDTLQDGWPQQVLTMQRNWIGRSTGMEITFPSANTEIYADGLTVYTTRADTLMGVTYVAVAAEHPLALKAAENNPELAAFIEECRIGSVAEADLATAEKKGMATGLFVKHPVTGEELPVWIANYVLMSYGSGAVMAVPAHDERDFEFANKFNLPIKQVI-DAKGADDADYSATEWQEWYGSKEGKLVNSGEFDGLEFQAAFDAFLAKLEPQGLANSKVQFRLRDWGVSRQRYWGCPIPMINCDTCGQVTVPEDQLPVVLPTDVVPDGSGNPLNKMPEFYETKCPCCGGDARRETDTLDTFVESSWYYARYASPDFTGGIVKPEAAKNWLPVNQYIGGVEHAILHLLYARFFHKLMHDEGVVQGNEPFTNLLTQGMVLADTFYREAENGKKTWFNPANIELERDEKGRIISAKYSGDGQEVIIGGQEKMSKSKNNGIDPQAIIDQYGADTARVFMMFAAPPDQSLEWSDAGVEGANRFLKRVWRLVASFLEKGNSATAIDKANLSKDAQDLRRKTHETIQKVSDDIERRHAFNTAIAALMELLNASNKFEAKDDNDVAVEREAITTLLTLLAPFAPHLSQTLLAQFGT--DLTEATFPEVDASALTRNTQTIVVQVNGKLRGKLEVSVDISKDELLAQAKALPEVQQFLT-GPTKKEIVVPNKLVNLVV 4ARC Chain:A ((21-879)) -----MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYPSGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTYDNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAV------------------------------EIPQWFIKITAYADELLNDLDKL-DHWPDTVKTMQRNWIGRSEGVEITFNVNDY---DNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNPELAAFIDE----------------KGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGAVMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDL-----SQQAL-TEKGVLFNSGEFNGLDHEAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQLPVILPEDVVMDGITSPIKADPEWAKTTV--NGMPALRETDTFDTFMESSWYYARYTCPQYKEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQGMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKD-AAGHELVYTGMSKMSKSKNNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTAKGDVAA-LNVDALTENQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAPTDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVEDSTLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLNLVV

General information: TITO was launched using: RESULT: Template: 4ARC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 4198 -137532 -32.76 -170.00 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -32.76 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.580

(partial model without unconserved sides chains): PDB file : Tito_4ARC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ARC-query.scw PDB file : Tito_Scwrl_4ARC.pdb: