Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKPLSSRFLFKSFGMVAVCMSLAACGDKAWWSNNDEPELESQQI-----KRLIPSRVHDRESWAKDIDDIMKD--LDI-----PKTKQNVCSIVAVVDQESNFV-ANPQVPGLGQKAVEEVSTRLNEKFEDKLGKTIGGTIAGYFEEVLRTQPSPDNNYMSQMRKVKTEKDLDLLYREIFDFMAKHYHVSALTGAAKLVGQDIGEKMNPITTLGSMQVHINYAKANKRS----SMNTAALRDDLYTEYGGLYYGIHRLMVYPADYDKAIYRFADYNSGMYSSRNAAFQKMLKELTDKDISLDGDLLLYTKDGDPRATQSESEKELITVFASNNVLVTPRQIRDDLKLEKEKKFESTQTYIALTKLYKSKTGKEPLYAIMPQVVISGPKLSR-DYNTNWYATRVNGRYETCMQRAKRIRL----------- 4PG7 Chain:A ((20-446)) HMKKLNIALL--GLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILVRDKSKKRPLNISQYHLTE-DVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVITANKDLLAVHLKLLEDLAEENGVALKFEASVAGGPNNISKFMGIL---NGTSNFILSKMTKEQTT------FEEALDEAKRLGFAEA-DPTDDVEGVDAARKVV-ITSYLSFNQVIKLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAAVEDEYNAIYVIGDAV---------GDTMFYGKGAGSLATGSAVVSDLLNVALFFESTLPPHF---ELKTDKTREMEKSNFFVVVNHVKGS---IENFENELKAILPFHRSLRVANYDNQSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV

General information: TITO was launched using: RESULT: Template: 4PG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1848 88918 48.12 238.38 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 48.12 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.148

(partial model without unconserved sides chains): PDB file : Tito_4PG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4PG7-query.scw PDB file : Tito_Scwrl_4PG7.pdb: