Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDAKMNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEE-IGRRMREEGLRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLFEKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDENGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGVEILDAIRRK 4M8L Chain:A ((12-204)) ------LKKLAATEAAKSITTEITLGVGTGSTVGFLIEELVNYRDKIK----TVVSSSEDSTRKLKALGFDVVDLNYAGEIDLYIDGADECNNHKELIKGGGAALTREKICVAAAKKFICIIDESKKVNTLGNFPLPIEVIPMARSYIARQIVKLGGQPVYR-----EQTITDNGNVILDVYNLKIDNPLKLETELNQITGVVTNGIF------------------------

General information: TITO was launched using: RESULT: Template: 4M8L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1065 16515 15.51 86.02 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 15.51 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_4M8L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M8L-query.scw PDB file : Tito_Scwrl_4M8L.pdb: