Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESAELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNCMFAPNVQLY-TATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGAGSVVTKSFPD-NVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDTVTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNKMKEE 2WLG Chain:B ((101-190)) -----------------------------------------------------------------------------------------------ISIGKDCMLAHGYEIRNTDMHPIYSLENGERINHGKDVIIGNHVWLGRNVTILKGVCIPNNVVVGSHTVLYKSFKEPNCVIAGSPAKIVK-------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2WLG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 376 -31886 -84.80 -362.34 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -84.80 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.659

(partial model without unconserved sides chains): PDB file : Tito_2WLG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WLG-query.scw PDB file : Tito_Scwrl_2WLG.pdb: