Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLKGASPMKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHLYHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMESQCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLSDILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKAANYNGVET-YFLEDTAQAGTELYELA---KGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGTISPLQMASQAVKKFGTVQVTGVYMTP-ASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYAMIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP 4EJ6 Chain:A ((21-362)) -----QSMMKAVRLESVGNISVRNVGIP-EPGPDDLLVKVEACGICGTDRHLLHGEFP-STPPVTLGHEFCGIVVEAGSAVRDIAPGARITGDPNISCGRCPQCQAGRVNLCRNLR-------AIGIH-----RDGGFAEYVLVP--RKQAFEIPLT-LDPVHGA-FCEPLACCLHGVDLSGIKAGSTVAILGGGVIGLLTVQLARLAGATTVILSTRQATKRRLAEEV-GATATVDPSAG-DVVEAIAGPVGLVPGGVDVVIECAGVA--------------------ETVKQSTRLAKAGGTVVILGVLPQGEKVEIEPFDILFRELRVLGSFI-NPFVHRRAADLVATGAIEIDRMISRRISLDEAPDVISNPAAAG--EVKVLVIP

General information: TITO was launched using: RESULT: Template: 4EJ6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2030 -187325 -92.28 -555.86 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -92.28 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.449

(partial model without unconserved sides chains): PDB file : Tito_4EJ6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EJ6-query.scw PDB file : Tito_Scwrl_4EJ6.pdb: