Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTRRKPMKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLLESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEEGNALLTKKTLES-QV-LTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKK---SGFYFEWKNLENYFLE---TRVPLFFLGDFNNPVDSQGYYT--------------------VRESCLLLQDSYVVANEKGKAATVEKKIDGWEQNTEKLRIDFIFVPEGMQ--VKKYQRIFDGIDSPIISDHYGVEIELDVNE 2MYI Chain:A ((1-256)) -------MRIISVNVNGIQT----AVERGLLSWLQAQNADVICLQDTRASAFELD-D--------------------P------AYQLDG--YFLYACEA---EVPAQGGVALYSRLQPKAVITGLGFET-------ADRYGRY--LQADFDKVSIATLLLPSGQNGDEDLNQKFKLMDDFARYLDKQRRKRREYIYCGSLYVAQQKLDIKNWRDSQQSPGFLAPERAWMDEIVGN-MGYVDALREVSREGDQYSWWPDNEQAEMLNLGWRFDYQLLTPGLRRFVRSARLP----RQPRFSQHAPLIVDYDWT-

General information: TITO was launched using: RESULT: Template: 2MYI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1094 -21465 -19.62 -94.98 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -19.62 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.216

(partial model without unconserved sides chains): PDB file : Tito_2MYI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MYI-query.scw PDB file : Tito_Scwrl_2MYI.pdb: