Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYTGISANERKEGARNMEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNEKRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHGGAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTND-DTWRVTVQG-ISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLN-KDTTPTGEVVSVSGTAFDFQRPKKLGEVFASDFRQSMIV--GGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVKTDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVREFVTEYKINIKK 1NSX Chain:B ((2-339)) -----------------SIKIRDFGLGSDLISLTNKAGVTISFTNLGARIVDWQKDG----KHLILGFDSAKEYLEKDAYPGATVGPTAGRIKDGLVKISGKDYILNQNE-GPQTLHGGEESIHTKLWTYEVTDLGAEVQVKFSLVSNDGTNGYPGKIEMSVTHSF-DDDNKWKIHYEAIS-DKDTVFNPTGNVYFNLNGDASESVENHGLRLAASRFVPLKDQTEIVRGDIVDIKNTDLDFRQEKQLSNAFNSN--MEQVQLVKGIDHPFLLDQLGLDKEQARLTLD--DTSISVFTDQPSIVIFTANFGDLGTLYHEKKQVHHGGITFECQVSPGSEQIPELGDISLKAGEKYQATTIYSLHTK-

General information: TITO was launched using: RESULT: Template: 1NSX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2076 39366 18.96 118.21 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : 18.96 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.478

(partial model without unconserved sides chains): PDB file : Tito_1NSX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NSX-query.scw PDB file : Tito_Scwrl_1NSX.pdb: