Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------------------------------MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQSFKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTEAIQ----------DPTVKSVAISFDDGPGATTTPQLLRILKEKTCMSLFSS-------------------------------------------------------------------------------------------------------------------------------------------------------------- 5JP6 Chain:A ((23-379)) MSQIGSSVRQAVSDNQSAQTLVEWENSEANPEALFANWRHEFMVDSSKRESMKTELCKELQALP------AQDLTLFENEIRDENNRALVSG--CKEELLAQVDEHFDEQRESMSVPGHALKAVQSRNSFRFPDNTQKRDMSNGYMAVRGDVARKEVVLTFDDGPHGLYTDAILRALKEVNAKAMFFATGKSVRTNPEALKRVAADGHVIGSHSITLTFDEAAAEVRGGHQAVFDVLGWVDPVFRFYGETSKDLKAFLKTKSTGEFAWNIESDDWRTQSNEQLLARVLANVESQGRGIVLFHDIQRRTAEIMPQFLRELYNRGYSVVLLTAADPSAKYNSKLVKRK

General information: TITO was launched using: RESULT: Template: 5JP6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 381 40632 106.65 338.60 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 106.65 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.220

(partial model without unconserved sides chains): PDB file : Tito_5JP6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JP6-query.scw PDB file : Tito_Scwrl_5JP6.pdb: