Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLPLANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDRKMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDAYSFHATEESLDETYKDYEKAYTEVFKRCGLEFR-SIIGDGGAMGGKDSKEFMAISEIGEDTICYSTES-DYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQKIIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVGPVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDPSPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLSAIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDRNERAGVKFADSDLIGCPIRITVGKKAV-DGIVEVKIKKTGEMVEVRKEELVNTLPILLNQGE 2I4N Chain:C ((21-457)) MRLSRFFLPILKENPKEAEIVSHRLMLRAGMLRQEAAGIYAWLPLGHRVLKKIEQIVREEQNRAGAIELLMPTLQLADLWRESGRYDAYGPEMLRIADRHKRELLYGPTNEEMITEIFRAYIKSYKSLPLNLYHIQWKFRDEQRPRFGVMRGREFLMKDAYSFDVDEAGARKSYNKMFVAYLRTFARMGLKAIPMRAET-GPIGGDLSHEFIVLAETGESGVYIDRDVLNLPVP-D------------------------------ENVDYD-------------------------GDLTPIIKQWTSVYA---------------------------------AT-----------------EDVHEP------------------ARYESEVPEA--NRLNTRGIEVGQIFYFGTKYSDSMKANVTGPDGTDAPIHGGSYGVGVSRLLGAIIEACHDDNGIIWPEAVAPFRVTILNLKQGDAATDAACDQLYRELSAKGVDVLYDDTDQRAGAKFATADLIGIPWQIHVGPRGLAEGKVELKRRSDGARENLALADVVARL--------

General information: TITO was launched using: RESULT: Template: 2I4N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 2362 -57900 -24.51 -133.41 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain C : 0.75 3D Compatibility (PKB) : -24.51 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.483

(partial model without unconserved sides chains): PDB file : Tito_2I4N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I4N-query.scw PDB file : Tito_Scwrl_2I4N.pdb: