Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQRELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQYIVKSPKTGKIL-KLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNI--DFPEGLWQKYCFVLYQNRRMKQPYLP--DIKLAQRLAEFRQVEMPIYIADYQALENEIKKISEHINGQ-------------SKKRQIKKELFDDFAEEL-----------VKDVEEFLINYRAFLRK---------DWQSIV----YEEVPVHPNAYFETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIPRIVRNGFLKVLRKEREAIQ----SADKKDRTVSKEKWQLVVKDI------------------QSGNVQETIDKWKGKHYRFTEAQKQALEYYQKLEESLRF--NDEARKYLKELL 2HCN Chain:A ((12-582)) LVNGVVRLLSKPWDTITTKAPEPPEGVKYVLNETTNWLWAFLAREKRPRMCSREEFIRKVNSNAAQWRSAREAVEDPKFWEMVDEEREAHLRGECHTCRAIWFMWLGARFLEFEALGFLNEDHWLGRKNSGGGVE---GLGLQKLGYILREVGTRPGGRIYADDTAGWDTRITRADLENEAKVLELLDGEHRRLARAIIELTYRSGQVVTYALNTFTNLAVQLVRMMEGEGVIGPDDVEKLTKGKGPKVRTWLSENGEERLSRMAVSGDDCVVKPLDDRFATSLHFLNAMSKVRKDIQEWKPSTGWYDWQQVPFCSN----HFTELIMKDGRTLVTPCRGQDELVGRARISPNVRDTACLAKSYAQMWLLLYFHRRDLRLMANAICSAVPVNWVPTGRTTWSIHAGGEWMTTEDMLEVWNRVWIEENEWMEDKTPVEKWSDVPYSGKREDIWCGSLIGTRARATWAENIQVAINQVRSIIGDEKYVDYMSSLK

General information: TITO was launched using: RESULT: Template: 2HCN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2094 94195 44.98 225.35 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 44.98 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.233

(partial model without unconserved sides chains): PDB file : Tito_2HCN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HCN-query.scw PDB file : Tito_Scwrl_2HCN.pdb: