Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGFTEETVRFKLDDSNKKE-ISETLTDVYASLNDKGYNPINQIVGYVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL 1PYS Chain:B ((400-474)) ---PPEAIPFRPEYANRLLGTSYPEAEQIAILKRLGC--------RVEGEGPTYRVTPPSHRLDLRL--EEDLVEEVARIQ-GYETIPL

General information: TITO was launched using: RESULT: Template: 1PYS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 293 785 2.68 10.61 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : 2.68 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.346

(partial model without unconserved sides chains): PDB file : Tito_1PYS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PYS-query.scw PDB file : Tito_Scwrl_1PYS.pdb: