Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLKTELVIFDWDGTLYNSVGQIVASLQHAAEEHKLTLTD-EAAKSIIGLGLPEVMQTLFPEVP-DLHDSILKAYGDHYIANST-NDAWFEGISELLHDLKAQGLKLAVATGKNRRGLDRVIAKTQSTHLFDVTR-AANE--TRSKPDPLMLQEILTVTGVSVEQAVMIGDSSYDLEMAQRLGMPRIGVGYGVHSV----EVLQQFQPLTIAKDVSELHNFLREYAKLSTVDVA 4EEK Chain:A ((25-249)) -DAPFDAVLFDLDGVLVESEGIIAQVWQSVLAERGLHLDLTEIAMYFTGQRFDGVLAYLAQQHDFVPPPDFLDVLETRFNAA-MTGVTAIEGAAETLRALRAAGVPFAIGSNSERGRLHLKLRVAGLTELAGEHIYDPSWVGGRGKPHPDLYTFAAQQLGILPERCVVIEDSVTGGAAGLAAGATLWGLLVPGHPHPDGAAALSRLGAARVLTSHAELRAALAEAGL-------

General information: TITO was launched using: RESULT: Template: 4EEK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1103 -10818 -9.81 -50.31 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -9.81 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.591

(partial model without unconserved sides chains): PDB file : Tito_4EEK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EEK-query.scw PDB file : Tito_Scwrl_4EEK.pdb: