Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHRQVLILASSQSLFQTVSVMVMTIGGLA-GANIANTPTLSTLPIASMFLGTALMMFPASILMAHIGRRNGFLFGAFLGVLGGIIASIGIF--YSSLSLLALGTLCVGAYQSFAQFYRFAASEVANDAFRSRAISWVLAGGIVAALIGPTLARFGGPLFQ--HLEY-------------IGSFLIISIISLVAMGILSS-LHIADTVEQKSDFTAGRPWQQIVFQPTYLVALFGAITGYGIMILGMTATPIAMRHSHHELGSITTVIQLHVLGMFLPSFFTGNLIARFGVLKIMFAGLLLFACYIGFALS---GI----QFTSF----AISLILLGVGWNFLFIGSTSLLTGTYSV-----------------AEKAKAQAINDMTVFVVGLICSFSAGALLDLFGW-KAMNIALIPWLVIAAASLFWLSQKREKQFV 4U4T Chain:A ((37-450)) ---NLWISVPCLLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGRRWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPKQKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWIWVPFLAIFTIAAWFGMNDLAT------------QLPVLKRGHLWIMSLLYLATFGSFIGFSAGFAMLSK-TQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGLLFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSDERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV---------------

General information: TITO was launched using: RESULT: Template: 4U4T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1729 -260458 -150.64 -725.51 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -150.64 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.300

(partial model without unconserved sides chains): PDB file : Tito_4U4T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4U4T-query.scw PDB file : Tito_Scwrl_4U4T.pdb: