Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNFLPAEEQLALIQRGTHEIISEE-DLLKKLKENRPLKIKAGFDPTAPDLHLGHTVLINKLKTFQDLGHEVTFLIGDYTAMIGDPTGKSATRPPLSREQVEANAKTYQEQVFKI----LDPNKTKVRFNSEWFNQKSAADLIQ-LASQQTVSRMLERDDFTKRYNNHQPIAIHEFLYPLVQGYDSIAL----EADVELGGTDQTFNLLMGRTLQSRYGQESQVCITVPILEGLDGVNKMSKSLG-NYIGVFD---APGAMYQKVLSMPDSLIERYFDLLSFK-SLDEIKALLDEIAAGRNPQEVKRILALELVERFHDAEAAANAHKSAGNRITEGEVPADTPEVTISR-------GEFG--GEIFIATILRVAGLNPNAAAAKDAVARGAVKVDWNVVD-ASFSVK----ENGT-FIIQSGKKAIARVTFTD 2JAN Chain:C ((5-422)) --------ILDELSWRGLIAQSTDLDTLAAE-AQRGPMTVYAGFDPTAPSLHAGHLVPLLTLRRFQRAGHRPIVLAGGATGMIG--------------DTVAEWTERIRGQLERFVDFDDSPMGAIVENNLEWTGSLSAIEFLRDIGKHFSVNVMLARDTIRRRLA-GEGISYTEFSYLLLQANDYVELHRRHGCTLQIGGADQWGNIIAGVRLVRQKLGATVHALTVPLVTAADGT-KFGKSTGGGSLWLDPQMTSPYAWYQYFVNTADADVIRYLRWF-TFLSADELAELEQATAQRPQQRAAQRRLASELTVLVHGEAATAAVEHASRALFGRGELA-RLDEATLAAALRETTVAELKPGSPDGIVDLLVASGLSASKGAARRTIHEGGVSVNNIRVDNEEWVPQSSDFLHGRWLVLRRGKRSIAGVER--

General information: TITO was launched using: RESULT: Template: 2JAN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1675 -1207 -0.72 -3.22 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain C : 0.72 3D Compatibility (PKB) : -0.72 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.434

(partial model without unconserved sides chains): PDB file : Tito_2JAN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JAN-query.scw PDB file : Tito_Scwrl_2JAN.pdb: