Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MMVFELVKHSPNALRRYMTDMNVSASALANLTNISESKINKSLVEVEVFKLSQLETISKVLFVPTVYLTTDNFVYERNTPELIEFRNHIDIPQDRYKENALVQEFCQVRDNFISVLSSLNEEPKAFDLRLSGTNAEEDAQTIIDYFGFYTHTKKTKNSDDYFNAWRDIVELKDVIVIDRGREKFGSDGMCLYFDTAPIITIFSSGQSQSRKLFTLIHEIVHLGLGSSVFDGRLLESDNSLEKYCDQVAGYVLAPKSIVAACFNEYLTIEENVILIRKQTKASKAAIAIQLKMLGLI--NQDQLTDYLEYIKPKENGGGFGSKKENMVLKYFGHSFVEKVMSAMWQE--RISSNTAKNILGFHKTSKPSAFKELQQKVF 4F3V Chain:A ((1-275)) GHMTDRLASLFESAVSMLPMSEARSLDLFTEITNYDESACDAWIGRIRCGD--------------TDRVTLFRAWYSRRNFGQLSGSVQISMSTL----NARIA----IGGLYGDITYPVTS-PLAITMGFAACEAAQ---------GNYADAMEALEAAPVAGSEHLVAWMKAVVYGAAER-------------WTDVIDQVKSAGKWPDKFLAGAAGVAHGVAAANL--ALFTEAERRLTEANDSPAG------------------------------EACARAIAWYLAMARRSQGNESAAVALLEWLQTTHPE--------------------PKVAAALKDPSYRLKTTTAEQIASRADPWDPGSVV-------

General information: TITO was launched using: RESULT: Template: 4F3V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1363 -2730 -2.00 -10.11 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -2.00 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.198

(partial model without unconserved sides chains): PDB file : Tito_4F3V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F3V-query.scw PDB file : Tito_Scwrl_4F3V.pdb: