Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSFMQQSRLKVILNMRHELREIFIKGCDDKIEKKGDN----------VGLS---FYAFFKNKNDNPELLMEAAHWWIMEHKLDHFEKAVKIKQLVLSEH 3HIM Chain:B ((13-72)) ---------------GTSKAAARIRAAAIEVFAAKGYGATTTREIAASLDMSPGAVYPHYKTKES---LLYAISLEGH----------------------

General information: TITO was launched using: RESULT: Template: 3HIM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 136 -5177 -38.06 -110.14 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : -38.06 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.680

(partial model without unconserved sides chains): PDB file : Tito_3HIM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HIM-query.scw PDB file : Tito_Scwrl_3HIM.pdb: