Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHSQPLLDCWNLDQILDDLNAHGFAIVNQAYSAEYHTQVAKECSHHFDEFREAGIQNGVVSTIRSDHILWINESLPVAEQHVETL-TSFCQHLNQAFFL-GIKEVEAHFACYNPGEFYALHRDNPQQ-----KNDRIMSTVYYLHPQWQDDWGGQLRLQDKNDIWHIITPEPNRLVIFQSNLLHEVLVSK-QQRLSITAWLRSGNSIWV 3DKQ Chain:C ((15-196)) -----------------LYFQGMLIEIPNVFSKQEVSHLREQLDAR--RWIDGNQ-------RKRN--QQLDKDDPVAVALGQQIMDRLLAHPQFVSAALPLQFYPPLFNRYQGGETFGYHIDNAIRSTPDGMIRTDLSATLFLSEP-ENYQGGELVIQDTYG-QQSIKLSAGSLVLYPSSSLHQVTPVLSGERTAAFMWLQSM-----

General information: TITO was launched using: RESULT: Template: 3DKQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 775 -6019 -7.77 -36.26 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain C : 0.62 3D Compatibility (PKB) : -7.77 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.522

(partial model without unconserved sides chains): PDB file : Tito_3DKQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DKQ-query.scw PDB file : Tito_Scwrl_3DKQ.pdb: