Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATRITNQKLSKSSRAWMREHLDDPFVKKAQKEGYRARAAYKLLEIQEKYKLIKPGMTVVDLGAAPGSWSQIAGKLVGSKGLVIASDILPMDALPDVTFLQGDFREEAVFEKL---LNILNGRQVDIVISDMAPNTSGNRAVDQPRQIYLCELALDFAQKVLGPNGQFVVKVFQGAGFDEFRKQVVDSFDVLKTAKPAASRARSKEVFLVGQGRKKALQ 3DOU Chain:A ((4-183)) ---------------------------------QLRSRAAFKLEFLLDRYRVVRKGDAVIEIGSSPGGWTQVLNSLA---RKIISIDLQEMEEIAGVRFIRCDIFKETIFDDIDRALREEGIEKVDDVVSDAMAKVSGIPSRDHAVSYQIGQRVMEIAVRYLRNGGNVLLKQFQGDMTNDFIAIWRKNFSSYKISKP------SSEIYIMFFGFKA---

General information: TITO was launched using: RESULT: Template: 3DOU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 887 -49562 -55.88 -289.84 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -55.88 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.595

(partial model without unconserved sides chains): PDB file : Tito_3DOU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DOU-query.scw PDB file : Tito_Scwrl_3DOU.pdb: