Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYTFDYLVFIGRFQPFHLAHMQTIEIALQQSRYVILALGSAQMERNIKNPFLAIEREQMILSNFSLDE--QKRIRFVHVVDVYNDEKWVKQVKSLINGVIEPNSKVGLIGHFKDESSYYLRLFPEWVM--VELD--SLKDSISATPMREAYYQGKIKTDAFPKGTIQFLEEFQNTPLYLQLQQKFLQADHSNIEETVSSHKE 1M8K Chain:C ((3-171)) ---TMRGLLVGRMQPFHRGALQVIKSILEEVDELIICIGSAQLSHSIRDPFTAGERVMMLTKALSENGIPASRYYIIPVQDIECNALWVGHIKMLT-----PPFDRVYSGNPLV-----QRLFSEDGYEVTAPPLFY-RDRYSGTEVRRRMLDDGDWRSLLPESVVEVIDEINGVERIKHLAK-------------------

General information: TITO was launched using: RESULT: Template: 1M8K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 790 -24659 -31.21 -151.28 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain C : 0.69 3D Compatibility (PKB) : -31.21 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_1M8K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1M8K-query.scw PDB file : Tito_Scwrl_1M8K.pdb: