Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVETAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVHQDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEVADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAVEVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGETLSLQEFAALSNAMSENMQ 1QAM Chain:A ((10-243)) -----------------------------QNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFTLELVQRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPK--------NQSYKIFGNIPYNISTDIIRKIVFD-SIADEIYLIVEYGFAKRLLNTKR-----SLALFLMAEVDISILSMVPREYFHPKPKVNSSLIRLNRKKSRI-SHKDKQKYNYFVMKWVNKEYKKIFTK------------NQFNNSLKHAGIDD---LNNISFEQFLSLFNSYKLFN-

General information: TITO was launched using: RESULT: Template: 1QAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1119 -159540 -142.57 -681.79 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -142.57 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.484

(partial model without unconserved sides chains): PDB file : Tito_1QAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QAM-query.scw PDB file : Tito_Scwrl_1QAM.pdb: