Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MITSDIKNA----DEYVRKVDKSQIPTCN------IMGVNIAAINMEWLLEYLEKNIDLL--HGDYICVSNVHTTVT------SFEEPDYCAVQNGGIMAIPDGGPLSTVGRKRGYKNMERTTGPSLM--GKIFKVTNEKGYRHFFYGSKQETLDLLEEKLKVYY-PGIQIAGMYSPPFRPLTEEEDK--AVIKMINAVKPDFV-WIGLGAPKQEKWMAEHQGKIDGLMIGVGAGFDYYAGNIQRAPQWMQKWNLEWMYRLIQDPKRLFYRYWHTNTKFILNAVIRRK 1NF2 Chain:A ((1-267)) MYRVFVFDLDGTLLNDNLEISEKDRRNIEKLSRKCYVVFASGRMLVSTLNVEKKYFKRTFPTIAYNGAIVYLPEEGVILNEKIPPEVAKDIIEYIKPLNVHWQAYIDDVLYSEKDNEEIKSYARHSNVDYRVEPNLSELVSKMGTTKLLLIDTPERLDELKEILSERFKDVVKVFKSFPTYLEIVPKNVDKGKALRFLRERMNWKKEEIVVFGDNENDLFMFE--------EAGLRVAMENAIEKVKEASDIVTLTNND----------SGVSYVLERISTDCLD------

General information: TITO was launched using: RESULT: Template: 1NF2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1233 13941 11.31 58.09 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 11.31 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.262

(partial model without unconserved sides chains): PDB file : Tito_1NF2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NF2-query.scw PDB file : Tito_Scwrl_1NF2.pdb: