Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKIAIIGGGIIGMTLASYLDTS-KF-NVALFDE-GTG-QATKASAGIISPWLS--------KRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLR-PEKDLAELAVLAEKRKEEAPEIGNI-EWLSPEQTSQALPLLKP-------A-VSLRISGGGRLDGKAYLEHLGQRLAQKQMEQIQ--AKVT-LHKKSDSWLIKGP--F-GEYTADRVILTPGPALKPLLQELGYAADIRPQKGQLLAFQTSYKMSQDWPVA-M-LDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKGLTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLTTGPYIGYLLANFLNTGHW-PT-EDYQKDISTYITKNPS 3AD8 Chain:B ((20-391)) SYDVVIVGGGGHGLATAYYLAKNHGITNVAVLEKGWLAGGNMARNTTIIRSNYLWDESAGIYEKSLKLWEELPEEL------------EYDFLFSQRGVLNLAHTLGDVRESIRRVEA----NKFNGVDAEWLTPEQVKEVCPIINTGDNIRYPVMGATYQPRAGIAKHDHVAWAFARKANEMG-VDIIQNCEVTGFLKD--GEKVTGVKTTRGTILAGKVALAGAGHSSVLAELAGFELPIQSHPLQALVSELFEP---VHPTVVMSNHIHVYVSQAHKGELVMGAGIDSYNGYGQRGAFHVIEEQMAAAVELFPI--FARAHVLRTWGGIVDTTMDASPIISKTP-IQNLYVNCGWGTGGFKGTPGAGYTLAHTIAHDEPHKLNAPFAL--ERFETG---

General information: TITO was launched using: RESULT: Template: 3AD8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2015 20007 9.93 58.84 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : 9.93 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_3AD8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3AD8-query.scw PDB file : Tito_Scwrl_3AD8.pdb: