Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------DVEPPGASVKPTEAARRRVF--KIDGEEFTPLETDCLIRIF--KNKKT-------VHALFVPPGTELDWRLDHNHYAVGIKHNCDIIIGWKPS--SWRIEGDKVSSLADGFSVVTTKSTD- 1VJ2 Chain:A ((13-126)) MILKRAYDVTPQKISTDKVRGVRKRVLIGLKDAPNFV------MRLFTVEPGGLIDRHSHPWEHEIFVLKG-KLTVLKEQG--EETVEEG-FYIF--VEPNEIHGFRNDTDSEV--EFLCLIPKEGGE

General information: TITO was launched using: RESULT: Template: 1VJ2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 297 7126 23.99 76.62 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 23.99 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.221

(partial model without unconserved sides chains): PDB file : Tito_1VJ2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VJ2-query.scw PDB file : Tito_Scwrl_1VJ2.pdb: